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One half of a brain was homogenized in 3 ml of lysate buffer (10 mM Tris-HCl, pH 7.8, ... l of lysate buffer containing 10 mM phenol, 18.2 mM l-epinephrine, ...l SDS-lysate-buffer (10 min 95°C, SDS lysis buffer: 1% SDS, 5 mM EDTA, 2 mM EGTA, 0.1 mM DTT, 10 mM Tris pH 7.5, protease inhibitors) according to a ...Decidua basalis tissue pooled from Day 10 pregnant rats was homogenized in lysate buffer (20 mM Tris base pH 7.5, 1% Nonidet P-40, 0.5% sodium deoxycholate, ...As a control to detect full-length cyclin-HA, transfected cell extract was added to lysate buffer alone (left lane). Following incubation of these mixed ...with one-half cell culture volume of 100 mM sodium phosphate buffer (pH 7.8) and once with. one-tenth cell culture volume of cell lysate buffer (100 mM ...The Sepharose beads were washed thrice with wash buffer (a 1:1 mixture of whole-cell lysate buffer and dilution buffer). Annexin V Apoptosis ...Note: When necessary, rabbit reticulocyte lysate or purified chaperones and cochaperones are added to Recovery Buffer just before use. ...
lysate buffer
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